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SUMMARY:Cytoskeleton Club - Paola Llinas "Molecular characterization of th
 e interaction of JIP3 and JIP4 adaptors with the kinesin heavy chain\, KIF
 5B : insights on the complexity of coiled coil interactions in kinesin-1 r
 egulation"
DTSTART:20260410T090000Z
DTEND:20260410T110000Z
DTSTAMP:20260711T234800Z
UID:indico-event-381@indico.i2bc.paris-saclay.fr
DESCRIPTION:Speaker: Paola Llinas\, MIKICA team\, I2BC\nTitle: Molecular c
 haracterization of the interaction of JIP3 and JIP4 adaptors with the kine
 sin heavy chain\, KIF5B : insights on the complexity of coiled coil intera
 ctions in kinesin-1 regulation\nAbstract: Intracellular transport is highl
 y regulated\; one key mechanism is the autoinhibition of molecular motors 
 and adaptor proteins. Although progress has been made in understanding how
  kinesin autoinhibition works\, crucial details about its activation—par
 ticularly how cargo-motor adaptors regulate it—are still unknown. JIP3 a
 nd JIP4 are adaptors that activate kinesin-1 and dynein-dynactin motors\, 
 enabling bidirectional transport along microtubules. I will talk about the
  characterization of the interaction between the kinesin-1 heavy chain\, K
 IF5B\, and both JIP3 and JIP4 (JIP3/4)\, using extensive site-directed mut
 agenesis and binding experiments associated with modelling. We determined 
 the regions of JIP3/4 and KIF5B required for interaction\, identify differ
 ences between JIP3 and JIP4 that can modulate this interaction and propose
  a mechanism in which an extended charged surface with multiple weak-affin
 ity sites would be needed to bind the kinesin-1 heavy chain for activation
 . It will be the occasion to talk about the complexity of coiled coil inte
 ractions in kinesin-1 regulation that are challenging for Alpha Fold predi
 ction.\n\nhttps://indico.i2bc.paris-saclay.fr/event/381/
LOCATION:B22-N0-001 - Salle de Conférences (I2BC CNRS Gif)
URL:https://indico.i2bc.paris-saclay.fr/event/381/
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