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SUMMARY:PhD defense -  Meng Wen (eq. Yamaichi - Genome Biology dpt)
DTSTART:20260715T120000Z
DTEND:20260715T160000Z
DTSTAMP:20260628T023100Z
UID:indico-event-413@indico.i2bc.paris-saclay.fr
DESCRIPTION:Title: “DNA transactions during the establishment of conjuga
 tive plasmid transfer”\n \nAbstract: The worldwide spread of antimicro
 bial resistance (AMR) has emerged as one of the most serious public health
  challenges. Bacteria can develop AMR by spontaneous mutations or acquirin
 g resistance genes through horizontal gene transfer such as transduction\,
  transformation\, and conjugation. Among them\, conjugation is widely cons
 idered to be the most effective pathway for the transmission of AMR genes 
 by conjugative plasmid. Indeed\, successful conjugative transfer of plasmi
 d which includes establishment in new host cell\, requires complex DNA tra
 nsaction\, such as plasmid DNA circularization\, double-strand DNA synthes
 is and prompt gene expression. However\, detailed molecular mechanisms of 
 this dynamic process remain elusive.\nIn my thesis\, I developed a new met
 hod\, ED-TA\, for achieving Elimination of Donor cells for Transconjugant 
 Analysis. ED-TA method enables genome-wide analysis of DNA reactions in re
 cipient cells during conjugation. Based on ED-TA\, I carried out RNAseq wh
 ich unveiled the transcriptome profiling of pESBL at early stages of conju
 gation. Additional genetic experiments collectively showed that the ssDNA 
 promoter in the leading region (the first fragment enters the recipient ce
 lls which encoded anti-defense systems) induces the expression of certain 
 plasmid genes in the early stages of conjugation\, and these genes play an
  important role in plasmid establishment in the recipient cell. These inno
 vations and insights will be helpful for future understanding of molecular
  mechanisms and subsequent development of targeted strategies to block the
  transmission of drug-resistant plasmids. In addition\, the SOS response i
 s believed to be a bacterial defense system against foreign DNA (such as c
 onjugative plasmids). However\, with emerging controversial results\, I fu
 rther investigated the biological significance of the SOS response and the
  regulation of SOS inhibitor during conjugation.\nMembers of the jury:Dr. 
 Sarah BIGOT (DR CNRS\, MMSB Lyon) Rapportrice & ExaminatriceDr. Bianca SCL
 AVI (DR CNRS\, IBPS Paris) Rapportrice & ExaminatriceDr. Olga SOUTOURINA (
 PR Univ Paris-Saclay\, I2BC) ExaminatriceDr. Laurent AUSSEL (PR Aix-Marsei
 lle Univ. LCB Marseille) Examinateur \n\nhttps://indico.i2bc.paris-saclay
 .fr/event/413/
LOCATION:B26 new seminar room (I2BC CNRS Gif)
URL:https://indico.i2bc.paris-saclay.fr/event/413/
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