PhD Defense - François Charon (eq. Noordermeer - Genome Biology dpt)

Title: Multi-omics dissection of allele-specific regulatory mechanisms at the imprinted Dlk1-Dio3 domain

Abstract: Genomic imprinting is an epigenetic mechanism that controls the parent-specific, monoallelic expression of ~150–200 genes in mammals, organized in 20 clusters. This regulation is primarily mediated by parent-specific DNA methylation at CpG-rich regions called Differentially Methylated Regions (DMRs). DNA methylation status at DMRs orchestrates a cascade of allelic regulatory mechanisms, which can vary between imprinted domains, including histone modifications, CTCF-mediated 3D genome architecture and long non-coding RNAs (lncRNAs). In my thesis project, I focused on two mostly unexplored layers; differential replication timing and nuclear lamina association. Genomic imprinting is an epigenetic mechanism that controls the parent-specific, monoallelic expression of ~150–200 genes in mammals, organized in 20 clusters. This regulation is primarily mediated by parent-specific DNA methylation at CpG-rich regions called Differentially Methylated Regions (DMRs). DNA methylation status at DMRs orchestrates a cascade of allelic regulatory mechanisms, which can vary between imprinted domains, including histone modifications, CTCF mediated 3D genome architecture and long non coding RNAs (lncRNAs). In my thesis project, I focused on two mostly unexplored layers; differential replication timing and nuclear lamina association.

Jury members:
Présidente : Fabienne MALAGNAC ; Professeur, Université Paris-Saclay
Rapporteur & Examinatrice : Céline MOREY ; Chargée de recherche, Université Paris-Cité
Rapporteur & Examinateur : Maxim GREENBERG ; Directeur de Recherche, Université Paris-Cité
Examinateur : Jop KIND ; Professeur, Radboud Universiteit
Examinateur : Laurent JOURNOT ; Directeur de Recherche, Université de Montpellier